MicroRNA profiling by array of human cancer cell lines placed in hypoxia for 0, 6, 16 and 24 hours

ABSTRACT: Hypoxia in the tumor microenvironment plays a crucial role in the evasion of immune surveillance by tumor cells. In this study, we aimed to identify the miRs regulated by hypoxia in NSCLC and melanoma and their putative involvement in the regulation of tumor susceptibility to antigen-specific cytotoxic T lymphocyte (CTL)-mediated killing. MicroRNA-210 (miR-210) was significantly induced in a hypoxia inducible factor 1 alpha (HIF-1_) dependent manner in NSCLC and melanoma cells. We show for the first time that miR-210 was expressed in hypoxic zones of human NSCLC tissues. Transfection of anti-miR-210 in both hypoxic NSCLC and melanoma cells resulted in a significant restoration of their susceptibility to autologous CTL-mediated lysis, independent of tumor cell recognition and CTL reactivity

ORGANISM(S): Homo sapiens

SUBMITTER: Noman MZ 

PROVIDER: S-ECPF-MTAB-1157 | biostudies-other | 2012 Sep

REPOSITORIES: biostudies-other