Unknown

Dataset Information

0

Epitope map of two polyclonal antibodies that recognize amyloid lesions in patients with Alzheimer's disease.


ABSTRACT: Two synthetic peptides with sequences identical with those of fragments of the extracellular domain of the Alzheimer's-disease amyloid precursor protein (APP) were used to raise antibodies. SP28 comprises positions 597-624 of the APP695 isoform, whereas SP41 extends towards the N-terminus (amino acids 584-624) and contains the entire SP28 peptide. Using e.l.i.s.a. and inhibition experiments we identified the two beta-turn-containing segments 602-607 and 617-624 as the epitopes recognized by anti-SP41 and anti-SP28 respectively. Both antibodies immunolabelled amyloid lesions in brains from Alzheimer's-disease patients and patients with related disorders, whereas they were unreactive in control brains. However, when probed on immunoblots, anti-SP28 failed to detect full-length APP from baculovirus-infected Sf9 cells, and anti-SP41 reacted weakly compared with other anti-APP antisera. The data suggest that these antibodies are directed to conformational epitopes not existent in the native molecules but present after alternative APP processing.

SUBMITTER: Ghiso J 

PROVIDER: S-EPMC1130811 | biostudies-other | 1992 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4568325 | biostudies-literature
| S-EPMC5767539 | biostudies-literature
| S-EPMC10789674 | biostudies-literature
2010-05-30 | GSE20447 | GEO
2021-11-24 | PXD025620 | Pride
2015-02-12 | E-GEOD-65851 | biostudies-arrayexpress
| S-EPMC3776966 | biostudies-literature
| S-EPMC5155461 | biostudies-literature
| S-EPMC4622072 | biostudies-literature
| S-EPMC4322079 | biostudies-other