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Stimulation of neutrophil adhesion by antibodies recognizing CD15 (Le(X)) and CD15-expressing carcinoembryonic antigen-related glycoprotein NCA-160.

ABSTRACT: The carbohydrate antigen, CD15 (Le(X)), and its sialylated derivative have recently been shown to be involved in the binding of neutrophils to the endothelial lectins, E-selectin and P-selectin. Neutrophil NCA-160, a carcinoembryonic antigen (CEA)-related glycoprotein, is the major carrier of CD15, which is also expressed on the common beta 2 chain of leucocyte integrins. Rabbit IgG antibodies directed against CEA, which cross-react with neutrophil NCAs, increase the adhesion of neutrophils to plastic. This effect is also observed with F(ab')2 and Fab antibody fragments and a monoclonal antibody (mAb) recognizing the same antigen. Anti-CD15 mAbs inhibit adhesion at higher concentrations, but augment adhesion at lower concentrations. Anti-CEA and anti-CD15 antibodies cause the homotypic adhesion of neutrophils demonstrable by light microscopy and flow cytometry. Anti-(integrin beta 2 chain) mAbs inhibit both adhesion to plastic and homotypic adhesion. These results suggest that binding of ligand to NCA-160 is able to trigger neutrophil adhesion events which have been shown to be integrin mediated. Anti-CD15 mAbs do not, however, induce a respiratory burst from neutrophils.

SUBMITTER: Stocks SC

PROVIDER: S-EPMC1132074 | biostudies-other | 1992 Nov

REPOSITORIES: biostudies-other

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Purification and characterization of monoclonal IgG antibodies recognizing Ebola virus glycoprotein.

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| S-EPMC6082671 | biostudies-literature

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2015-09-19 | GSE73208 | GEO

Elevation of neutrophil carcinoembryonic antigen-related cell adhesion molecule 1 associated with multiple inflammatory mediators was related to different clinical stages in ischemic stroke patients.

Project description:BackgroundWe aimed to analyze the level of carcinoembryonic antigen-related cell adhesion molecule 1 (CEACAM1) in neutrophils of ischemic stroke (IS) patients at different stages, together with its roles in neutrophils.Patients and methodsSixty-seven patients were classified into acute phase group (n = 19), subacute phase group (n = 28), and stable phase group (n = 20), and 20 healthy individuals who had received physical examination at the same time period as healthy control. We then analyzed the expression level of CEACAM1 and cell viability in CEACAM1 positive and CEACAM1 negative neutrophils by flow cytometry and the content of plasma CEACAM1, neutrophil gelatinase-associated lipocalin (NGAL), matrix metalloproteinases-9 (MMP-9) was measured using enzyme-linked immunosorbent assay (ELISA), while that of interleukin-10 (IL-10) and tumor necrosis factor (TNF) was determined using a Human Enhanced Sensitivity Flex set.ResultsCompared with healthy control, the percentage of CEACAM1 positive neutrophils in IS patients showed a significant increase, and a significant increase was also noticed in the content of plasma CEACAM1 at the subacute stage. Reduction in cell viability was observed in CEACAM1 positive neutrophils compared with CEACAM1 negative counterparts. There was a positive correlation between CEACAM1 expression rate in neutrophils and plasma CEACAM1 and IL-10 content in the subacute group. Compared with acute group and healthy control group, there was an instinct increase in the level of plasma MMP-9 and NGAL in subacute group.ConclusionsOur data showed that there was a rapid increase of CEACAM1 in neutrophils at the acute stage of IS. We speculated that CEACAM1 may serve as an inhibitory regulator involving in the progression of IS.

| S-EPMC9279952 | biostudies-literature

Quantifying Carcinoembryonic Antigen-like Cell Adhesion Molecule-Targeted Liposome Delivery Using Imaging Flow Cytometry.

Project description:Carcinoembryonic antigen-like cell adhesion molecules (CEACAMs) are human cell-surface proteins that can exhibit increased expression on tumor cells and are thus a potential target for novel tumor-seeking therapeutic delivery methods. We hypothesize that engineered nanoparticles containing a known interaction partner of CEACAM, Neisseria gonorrhoeae outer membrane protein Opa, can be used to deliver cargo to specific cellular targets. In this study, the cell association and uptake of protein-free liposomes and Opa proteoliposomes into CEACAM-expressing cells were measured using imaging flow cytometry. A size-dependent internalization of liposomes into HeLa cells was observed through endocytic pathways. Opa-dependent, CEACAM1-mediated uptake of liposomes into HeLa cells was observed, with limited colocalization with endosomal and lysosomal trafficking compartments. Given the overexpression of CEACAM1 on several distinct cancers and interest in using CEACAM1 as a component in treatment strategies, these results support further pursuit of investigating Opa-dependent specificity and the internalization mechanism for therapeutic delivery.

| S-EPMC6740330 | biostudies-literature

Cell-dependent regulation of vasculogenic mimicry by carcinoembryonic antigen cell adhesion molecule 1 (CEACAM1).

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| S-EPMC6997815 | biostudies-literature

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