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Evidence for acidity of prelysosomal autophagic/endocytic vacuoles (amphisomes).


ABSTRACT: [14C]Lactose electroinjected into isolated rat hepatocytes is normally autophagocytosed, transferred to lysosomes and degraded by lysosomal beta-galactosidase, but at high concentrations of asparagine the transfer is inhibited and lactose accumulates in prelysosomal autophagic/endocytic vacuoles (amphisomes). The accumulation can be prevented by addition of yeast beta-galactosidase, which is transferred to the lactose-containing vacuoles by endocytosis. Propylamine, a weak base capable of neutralizing acidic vacuoles, protects autophagocytosed lactose against both endogenous and exogenous beta-galactosidase, suggesting that amphisomes, like lysosomes, have an acidic internal environment.

SUBMITTER: Stromhaug PE 

PROVIDER: S-EPMC1132489 | biostudies-other | 1993 Apr

REPOSITORIES: biostudies-other

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