Unknown

Dataset Information

0

The esterolytic activity of epidermolytic toxins.


ABSTRACT: The two epidermolytic toxins were shown to have intrinsic N-t-butyloxycarbonyl-L-glutamic acid alpha-phenyl esterase activity. The activity was dependent on free toxin pKa values of 6.6 and 6.8 for ETA and ETB respectively. ETB incorporated 0.97 mol of radiolabelled di-isopropyl phosphorofluoridate/mol of protein with loss of esterolytic and epidermolytic activities. The correspondence of epidermolytic and esterolytic activities in ETA and ETB during thermal inactivation and reaction with di-isopropyl phosphorofluoridate, together with the inactivity of the mutant protein ETA S195G, demonstrates that the two activities are dependent on a single active serine residue in each protein.

SUBMITTER: Bailey CJ 

PROVIDER: S-EPMC1132713 | biostudies-other | 1992 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC3815381 | biostudies-literature
| S-EPMC4280536 | biostudies-literature
| S-EPMC6386544 | biostudies-literature
| S-EPMC5959882 | biostudies-literature
| S-EPMC6071219 | biostudies-literature
| S-EPMC3815414 | biostudies-literature
| S-EPMC5128750 | biostudies-literature
| S-EPMC3646422 | biostudies-literature
2020-06-11 | GSE144029 | GEO
| S-EPMC1253543 | biostudies-literature