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Undifferentiated F9 embryonal carcinoma cells produce a short-chain collagen molecule.

ABSTRACT: The undifferentiated F9 embryonal carcinoma cells produce a unique collagen that decreases in amount during retinoic acid-induced differentiation of F9 cells into basement-membrane parietal endoderm. A bacterial-collagenase-sensitive protein of approx. 60,000 Da was resolved on polyacrylamide-gel electrophoresis. After pepsin digestion, two pepsin-resistant fragments containing hydroxyproline were demonstrated, suggesting that a portion of the molecule has a stable triple helix. The mRNA from the undifferentiated F9 cells translates a collagenase-sensitive protein with a molecular mass consistent with the 60,000 Da collagenous protein produced by undifferentiated F8 cells.

SUBMITTER: Smith BD

PROVIDER: S-EPMC1135193 | biostudies-other | 1988 Oct

REPOSITORIES: biostudies-other

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F9 Embryonal Carcinoma cell line

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