Unknown

Dataset Information

0

A survey of the kinetic parameters of class C beta-lactamases. Cephalosporins and other beta-lactam compounds.


ABSTRACT: Various cephalosporins, cefoxitin, moxalactam, imipenem and aztreonam were studied as substrates of six class C beta-lactamases. Nitrocefin, cephaloridine, cefazolin, cephalothin and cephalexin were good substrates, with kcat. values ranging from 27 to 5000 s-1. Cefuroxime, cefotaxime and cefoxitin exhibited low kcat. values (0.010-1.7 s-1) and low Km values, which suggested a rate-limiting deacylation. Imipenem and aztreonam were even poorer substrates (kcat. 2 x 10(-4)-3 x 10(-2) s-1) and, in the presence of a reporter substrate, behaved as transient inactivators. With moxalactam, biphasic kinetics were observed, indicating a possible rearrangement of the acyl-enzyme.

SUBMITTER: Galleni M 

PROVIDER: S-EPMC1135199 | biostudies-other | 1988 Oct

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1135198 | biostudies-other
| S-EPMC1132494 | biostudies-other
| S-EPMC4018810 | biostudies-literature
| S-EPMC9712332 | biostudies-literature
| S-EPMC16209 | biostudies-literature
| S-EPMC1134852 | biostudies-other
| S-EPMC7598492 | biostudies-literature
| S-EPMC1154387 | biostudies-other
| S-EPMC1163343 | biostudies-other
| S-EPMC4476283 | biostudies-literature