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Fluoroaluminate mimics agonist application in single rat hepatocytes.


ABSTRACT: Single rat hepatocytes microinjected with the photoprotein aequorin were stimulated with glycogenolytic agonists or low concentrations of fluoroaluminate. Both protocols resulted in the generation of oscillations in cytosolic free Ca2+ levels from a resting value of approx. 200 nM and peaking at over 600 nM. However, oscillations induced by receptor-dependent agonists were more regular in both frequency and time course than those induced by direct activation of G-proteins. The role of G-proteins in the generation of repetitive free Ca2+ oscillations is discussed.

SUBMITTER: Woods NM 

PROVIDER: S-EPMC1136929 | biostudies-other | 1990 Jan

REPOSITORIES: biostudies-other

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