Evidence for intracellular sodium pumps in permeabilized Xenopus laevis oocytes.
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ABSTRACT: Ouabain binding was studied in Xenopus laevis oocytes permeabilized by detergents. The behaviour of markers showed that 10 microM-digitonin selectively disrupts the plasma membrane. In the presence of ATP, oocytes permeabilized at 10 microM-digitonin bound no more ouabain molecules than were required to abolish active 86Rb+ uptake in the intact cells. However, the ouabain binding capacity increased approx. 2-fold when inner membranes were disrupted by SDS or excess digitonin, as judged from the accompanying release of the lysosomal marker beta-hexosaminidase. The results suggest that oocytes have a large internal pool of functional sodium pumps.
SUBMITTER: Schmalzing G
PROVIDER: S-EPMC1138682 | biostudies-other | 1989 Jun
REPOSITORIES: biostudies-other
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