Unknown

Dataset Information

0

Lipocortin-independent effect of dexamethasone on phospholipase activity in a thymic epithelial cell line.


ABSTRACT: In the cloned rat thymic endocrine epithelial cell line TEA3A1, treatment with dexamethasone leads to decreased levels of prostaglandin E2, prostaglandin F2 alpha, and thromboxane B2. Dexamethasone treatment also leads to a decrease of both calcium-dependent and calcium-independent phospholipase A2 activity measured in a cell-free assay. Dexamethasone-treated cells also have increased levels of lipocortin-I, a putative modulator of phospholipase A2 activity. The property of calcium-dependent binding of lipocortin to the particulate fraction was used to prepare cytosolic and particulate subcellular fractions which contained phospholiphase A2 activity but no lipocortin-I. Dexamethasone decreased phospholipase A2 activity in both cytosolic and particulate fractions even in the absence of lipocortin, suggesting the presence of a lipocortin-independent mechanism.

SUBMITTER: Piltch A 

PROVIDER: S-EPMC1138839 | biostudies-other | 1989 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1599980 | biostudies-literature
| S-EPMC149924 | biostudies-literature
| S-EPMC3781041 | biostudies-literature
| S-EPMC10901246 | biostudies-literature
| S-EPMC5586285 | biostudies-literature
| S-EPMC6778193 | biostudies-literature
| S-EPMC6064927 | biostudies-literature
| S-EPMC5698406 | biostudies-literature
| S-EPMC3726151 | biostudies-literature
| S-EPMC8469018 | biostudies-literature