A high-performance-liquid-chromatographic method for the assay of coproporphyrinogen oxidase activity in rat liver.
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ABSTRACT: An h.p.l.c. method was developed for the assay of coproporphyrinogen oxidase activity in rat liver. The protoporphyrinogen IX formed is completely oxidized to protoporphyrin IX for separation and quantification by reversed-phase chromatography with mesoporphyrin as the internal standard. The Km of coproporphrinogen oxidase is 1.01 +/- 0.23 microM. The activities are 4.07 +/- 0.40 nmol of protoporphyrin IX/h per mg of mitochondrial protein and 224 +/- 19 nmol of protoporphyrin IX/h per g of liver tissue homogenate. The method is sensitive enough for measuring enzyme activity in small amounts of human tissue from needle biopsy.
SUBMITTER: Li F
PROVIDER: S-EPMC1147306 | biostudies-other | 1986 Oct
REPOSITORIES: biostudies-other
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