Unknown

Dataset Information

0

Slow passive diffusion of NAD+ between intact isolated plant mitochondria and suspending medium.


ABSTRACT: Isolated potato (Solanum tuberosum) tuber mitochondria purified by isopycnic centrifugation in density gradients of Percoll were found to be highly intact, to be devoid of extramitochondrial contaminations and to retain a high rate of O2 consumption. When suspended in a medium that avoided rupture of the outer membrane, intact purified mitochondria progressively lost their NAD+ content by passive diffusion. This led to a slow decrease of oxoglutarate-dependent O2 consumption by isolated mitochondria. Addition of NAD+ to the medium restored the initial State-3 rate of oxoglutarate oxidation. The rate of NAD+ accumulation in the matrix space was concentration-dependent, exhibited Michaelis-Menten kinetics and was strongly inhibited by the analogue N-4-azido-2-nitrophenyl-4-aminobutyryl-NAD+.

SUBMITTER: Neuburger M 

PROVIDER: S-EPMC1152522 | biostudies-other | 1983 Nov

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC8620152 | biostudies-literature
| S-EPMC3855722 | biostudies-literature
| S-EPMC4004954 | biostudies-literature
2020-11-01 | GSE151376 | GEO
| S-EPMC1154021 | biostudies-other
| S-EPMC4937448 | biostudies-literature
| S-EPMC4646015 | biostudies-literature
| S-EPMC6497751 | biostudies-literature
| S-EPMC1395316 | biostudies-literature
| S-EPMC3371254 | biostudies-literature