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Inhibition by heparin-modulated antithrombin III of amidolysis catalysed by m beta-acrosin.


ABSTRACT: Purified m beta-acrosin catalysed amidolysis of several p-nitroanilides with C-terminal arginine residues. Antithrombin III inhibited amidolysis catalysed by the enzyme. This effect of antithrombin III was potentiated by heparin, and to a modest extent by heparan sulphate, cellulose sulphate, dextran sulphate and xylan sulphate. De-N-sulphated heparin, de-N-sulphated N-acetylated heparin, heparin of low relative molecular mass, chondroitin 4-sulphate, chondroitin 6-sulphate, dermatan sulphate and hyaluronic acid were ineffective.

SUBMITTER: Long WF 

PROVIDER: S-EPMC1153274 | biostudies-other | 1984 Feb

REPOSITORIES: biostudies-other

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