Unknown

Dataset Information

0

A macromolecular inhibitor of the antizyme to ornithine decarboxylase.


ABSTRACT: A macromolecular factor that inhibits the activity of the antizyme to ornithine decarboxylase (ODC) was found in rat liver extracts. The factor, 'antizyme inhibitor', was heat-labile, non diffusable and of similar molecular size to ODC. The antizyme inhibitor re-activated ODC that had been inactivated by antizyme, apparently by replacing ODC in a complex with antizyme. Therefore the antizyme inhibitor can be used to assay the amount of inactive ODC-antizyme complex formed in vitro. When assayed by this method, the complex was shown to be eluted before ODC from a Sephadex G-100 column. Significant increase in ODC activity was observed when the antizyme inhibitor was added to crude liver extracts from rats that had been injected with 1,3-diaminopropane to cause decay of ODC activity, suggesting the presence of inactive ODC-antizyme complex in the extracts.

SUBMITTER: Fujita K 

PROVIDER: S-EPMC1158403 | biostudies-other | 1982 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4066113 | biostudies-literature
| S-EPMC3084279 | biostudies-literature
| S-EPMC3170320 | biostudies-literature
| S-EPMC18314 | biostudies-literature
| S-EPMC3156769 | biostudies-literature
| S-EPMC8094758 | biostudies-literature
| S-EPMC3207831 | biostudies-literature
| S-EPMC5349141 | biostudies-literature
| S-EPMC1220902 | biostudies-other
| S-EPMC6377119 | biostudies-literature