Unknown

Dataset Information

0

A high-affinity oestrogen-binding protein in cockerel liver cytosol.


ABSTRACT: In contrast with several earlier reports, cytosol from cockerel liver contains a significant concentration of a protein that binds oestradiol with high affinity. To demonstrate the activity, certain alterations in the conventional method of preparation of cytosol must be made. Homogenization in sucrose-containing buffer at pH 8.4 in the presence of proteinase inhibitors and rapid fractionation of the cytosol with (NH4)2SO4 enables demonstration of a single class of oestradiol-binding sites with a Kd of about 1 nM and specificity only for oestrogens. The concentration is about 300 sites per cell in liver from 2-week-old cockerels. Oestradiol treatment in vivo decreases the number of exchangeable cytosol oestradiol-binding sites by about 80% for 1--4h, after which time it is gradually restored. Gel filtration of the cytosol preparation in the presence of high salt concentrations reveals that most of the oestradiol-binding activity is in high-molecular-weight aggregates, but a mild trypsin treatment generates a specific binding protein with an approximate mol.wt. of 40 000. This protein may be an oestrogen receptor.

SUBMITTER: Lazier CB 

PROVIDER: S-EPMC1161059 | biostudies-other | 1979 May

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1158596 | biostudies-other
| S-EPMC1162126 | biostudies-other
| S-EPMC2873402 | biostudies-literature
| S-EPMC5346138 | biostudies-literature
| S-EPMC8811889 | biostudies-literature
| S-EPMC1219287 | biostudies-other
| S-EPMC31124 | biostudies-literature
| S-EPMC7157921 | biostudies-literature
| S-EPMC2930565 | biostudies-literature
| S-EPMC8941649 | biostudies-literature