Unknown

Dataset Information

0

A comparison of methods for the measurement of protein turnover in vivo.


ABSTRACT: Steady-state rates of turnover of two single proteins were measured in vivo by two independent methods. The fractional rate of synthesis of liver ornithine aminotransferase, measured by a continuous infusion of L-[2,6-3H]tyrosine, was 0.42 day-1, whereas in the same animals the fractional rate of degradation measured by loss of radioactivity from amino acids labelled via [14C]bicarbonate was 0.40 day-1. The agreement between methods confirms the reliability of each method for the study of hepatic protein turnover. In contrast, [14C]bicarbonate-labelled amino acids are extensively reutilized in muscle, and are therefore unsuitable for measuring rates of muscle protein breakdown.

SUBMITTER: MacDonald ML 

PROVIDER: S-EPMC1161786 | biostudies-other | 1979 Nov

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC2843406 | biostudies-literature
| S-EPMC5738149 | biostudies-literature
| S-EPMC6812571 | biostudies-literature
| S-EPMC3090248 | biostudies-literature
| S-EPMC5031629 | biostudies-literature
| S-EPMC1172485 | biostudies-other
| S-EPMC1153017 | biostudies-other
| S-EPMC4321859 | biostudies-literature
| S-EPMC1165512 | biostudies-other
| S-EPMC5008858 | biostudies-literature