Ontology highlight
ABSTRACT:
SUBMITTER: Tomazela DM
PROVIDER: S-EPMC2843406 | biostudies-literature | 2010 Mar
REPOSITORIES: biostudies-literature
Analytical chemistry 20100301 6
We describe a method to measure protein synthesis and catabolism in humans without prior purification and use the method to measure the turnover of surfactant protein-B (SP-B). SP-B, a lung-specific, hydrophobic protein essential for fetal-neonatal respiratory transition, is present in only picomolar quantities in tracheal aspirate samples and difficult to isolate for dynamic turnover studies using traditional in vivo tracer techniques. Using infusion of [5,5,5-(2)H(3)] leucine and a targeted pr ...[more]