1,2-diacylglycerol kinase of human erythrocyte membranes. Assay with endogenously generated substrate.
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ABSTRACT: 1,2-Diacylglycerol kinase activity was measured in human erythrocyte membranes using an assay procedure in which the substrate was generated endogenously, either by treatment with a bacterial phospholipase C or by incubation with Ca24, which activates a membrane-bound polyphosphoinositide phosphodiesterase. The properties of 1,2-diacylglycerol kinase were broadly similar to those described previously, except that in the present work maximum activities were higher and there was evidence for a double pH optimum.
SUBMITTER: Allan D
PROVIDER: S-EPMC1162263 | biostudies-other | 1980 Nov
REPOSITORIES: biostudies-other
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