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1,2-diacylglycerol kinase of human erythrocyte membranes. Assay with endogenously generated substrate.


ABSTRACT: 1,2-Diacylglycerol kinase activity was measured in human erythrocyte membranes using an assay procedure in which the substrate was generated endogenously, either by treatment with a bacterial phospholipase C or by incubation with Ca24, which activates a membrane-bound polyphosphoinositide phosphodiesterase. The properties of 1,2-diacylglycerol kinase were broadly similar to those described previously, except that in the present work maximum activities were higher and there was evidence for a double pH optimum.

SUBMITTER: Allan D 

PROVIDER: S-EPMC1162263 | biostudies-other | 1980 Nov

REPOSITORIES: biostudies-other

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