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Fluorimetric assays for cathepsin B and cathepsin H with methylcoumarylamide substrates.


ABSTRACT: Benzyloxycarbonyl-phenylalanyl-arginine 4-methyl-7-coumarylamide was found to be an excellent substrate for the fluorimetric assay of cathepsin B, and arginine 4-methyl-7-coumarylamide for cathepsin H. Procedures were developed that are very convenient, and avoid the hazards associated with the use of naphthylamides.

SUBMITTER: Barrett AJ 

PROVIDER: S-EPMC1162479 | biostudies-other | 1980 Jun

REPOSITORIES: biostudies-other

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