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Studies of pyruvate-water isotope exchange catalysed by erythrocytes and proteins.

ABSTRACT: Erythrocyte suspensions in buffer made with 2H2O catalyse the exchange of pyruvate protons. This process can be easly observed by spin-echo proton magnetic resonance. The dominant exchange process is shown to be due to the formation of Schiff-base links between pyruvate and amino groups of haemoglobin. Other proteins with free alpha-amino groups also catalyse the exchange. The pH*-dependence of the exchange rate due to hen-egg-white-lysozyme reflects the dissociation of the alpha-amino group.

SUBMITTER: Simpson RJ

PROVIDER: S-EPMC1162619 | biostudies-other | 1981 Feb

REPOSITORIES: biostudies-other

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