Isolation of an actin-binding fragment of fibronectin.
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ABSTRACT: We have identified a specific actin-binding site in the adhesive glycoprotein fibronectin, isolated from chicken fibroblasts. Affinity chromatography of fragments, released from fibronectin by limited proteolysis with trypsin, chymotrypsin and subtilisin, on actin-Sepharose and other protein-Sepharose columns was used to locate the binding site. A 27 000-mol.wt. subtilisin-digest fragment bound efficiently to actin. The results suggest that the actin-binding site is close to, but not identical with, the reported collagen-binding site.
SUBMITTER: Keski-Oja J
PROVIDER: S-EPMC1162640 | biostudies-other | 1981 Feb
REPOSITORIES: biostudies-other
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