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The incorporation in vitro of sulphate ions into synaptic-membrane glycoproteins.


ABSTRACT: Synaptosomes from sheep brain cortex were incubated with carrier-free Na235SO4 and the synaptic plasma membranes were isolated. The membranes were free of contamination from cytosol, mitochondria and microsomal material and accounted for 30% of the radioactivity present in the synaptosomal particulate fraction. Control experiments demonstrated that the radioactivity present in the preparation was not due to non-specific binding of sulphate ions. The synaptic membranes contained at least six 35S-containing protein bands with molecular weights between 160 000 and 16 000. Analysis showed that the radioactivity was located in the carbohydrate moiety of a glycopeptide.

SUBMITTER: White CJ 

PROVIDER: S-EPMC1163545 | biostudies-other | 1981 Nov

REPOSITORIES: biostudies-other

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