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Isolation of albumin from whole human plasma and fractionation of albumin-depleted plasma.


ABSTRACT: The dye Cibacron Blue F-3-GA was conjugated to Sepharose to provide an affinity column for serum albumin. Passage of whole human plasma through a column of Cibacron Blue-Sepharose results in the removal of approx. 98% of the albumin. The latter can be quantitatively recovered by desorption with NaSCN. Albumin-depleted plasma can be readily resolved into discrete fractions by a combination of conventional biochemical techniques. In particular, the resolution of plasma proteins with properties similar to those of native human plasma albumin can readily be accomplished by ion-exchange chromatography of the Sepharose-dye-treated plasma on DEAE-cellulose.

SUBMITTER: Travis J 

PROVIDER: S-EPMC1163854 | biostudies-other | 1976 Aug

REPOSITORIES: biostudies-other

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