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Purification and properties of bovine pituitary follitropin.

ABSTRACT: A reproducible procedure was developed for the purification of follitropin from frozen bovine pituitary glands. The method involved precipitation with (NH4)2SO4 and acetone, followed by ion-exchange column chromatography on CM-cellulose and DEAE-cellulose and gel filtration on Sephadex G-100. A specific radioligand-receptor assay for follitropin was used to locate the activity in eluates after column chromatography and gel filtration. The potency of the highly purified bovine follitropin as measured by Steelman-Pohley bioassay was 164 times that of NIH-FSH-S1 standard preparation. They yield of bovine follitropin was 2.9 mg/kg of frozen pituitary glands. Electrophoretically, bovine follitropin was more acidic in nature and migrated further towards the anode than lutropin and thyrotropin. The elution volume of bovine follitropin by gel filtration on Sephadex G-100 was very similar to that of bovine lutropin. The amino acid composition of bovine follitropin was similar to that of sheep and human follitropin, being rich in lysine, aspartic acid, threonine, serine, glutamic acid and half-cystine.

SUBMITTER: Cheng KW

PROVIDER: S-EPMC1164165 | biostudies-other | 1976 Dec

REPOSITORIES: biostudies-other

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Primary structure of the ovine pituitary follitropin alpha-subunit.

Project description:All the tryptic peptides of reduced and aminoethylated alpha-subunit of ovine pituitary follitropin were isolated. From their their composition and partial sequence analysis of the N- and C-termini of the oxidized protein and reliance on homology with the sequence of lutropin alpha-subunit, an entire structure for the follitropin alpha-subunit has been proposed. The structure of the alpha-subunits from the two ovine hormones are identical.

| S-EPMC1163164 | biostudies-other

Primary structure of the ovine pituitary follitropin beta-subunit.

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| S-EPMC1163165 | biostudies-other

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Bovine pituitary LC-MS/MS

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2022-08-11 | PXD009619 | Pride

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| S-EPMC1148789 | biostudies-other

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