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Glycosylation in vitro of an asparagine sequon catalysed by preparations of yeast cell membranes.


ABSTRACT: Preparations of yeast cell membranes can catalyse in vitro the N-acetyl-beta-D-glucosaminylation of the asparagine sequon at residues 34--36 of bovine pancreatic ribonuclease A. The relevant glycopeptides were isolated from tryptic hydrolysates of the glycosylated ribonuclease and analysed. The donor used was UDP-N-acetyl-D-glucosamine, although the mechanism of the transfer is unknown. Mn2+ ions at concentrations of 25 mM double the activity of the enzymic transfer.

SUBMITTER: Khalkhali Z 

PROVIDER: S-EPMC1164198 | biostudies-other | 1976 Oct

REPOSITORIES: biostudies-other

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