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An improved micro-method for avidin assay.

ABSTRACT: A sensitive method for avidin assay was devised. Tritiated biotin is bound to avidin and this complex is then bound to bentonite. Radioactivity is converted into a gas form by combustion and counted in an automatic proportional counter with 55% efficiency and background of 3.7c.p.m. The sensitivity is 1-2ng of avidin.

SUBMITTER: Elo H

PROVIDER: S-EPMC1167978 | biostudies-other | 1974 Apr

REPOSITORIES: biostudies-other

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