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Isolation and characterization of mitochondrial deoxyribonucleic acid of Acanthamoeba castellanii.

ABSTRACT: DNA was prepared from isolated mitochondria of Acanthamoeba castellanii and was shown to behave as a single component in density gradients, on ;melting' and on renaturation. From measurements of renaturation kinetics, sedimentation coefficient and electron micrographs the genome size of the mitochondrial DNA was calculated to be about 3.4x10(7) daltons. A small proportion of the preparations could be isolated as relaxed circular molecules of mean contour length 16.2mum.

SUBMITTER: Hettiarachchy NS

PROVIDER: S-EPMC1168062 | biostudies-other | 1974 Jul

REPOSITORIES: biostudies-other

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