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Inhibition of protein synthesis in reticulocyte lysates by trichodermin.

ABSTRACT: 1. The effect of trichodermin as an inhibitor of eukaryotic protein synthesis was studied in a reticulocyte cell-free system. 2. Trichodermin at a concentration of 25 mug/ml inhibits total protein synthesis instantaneously and stabilizes polyribosome profiles. Conversely, at a concentration of 0.25 mug/ml the drug inhibits total protein synthesis by only 70-75% and allows 30-35% breakdown of the polyribosomes in the system. These effects were compared with those produced by two other drugs (pactamycin and anisomycin) examined under conditions identical with those used for trichodermin.

SUBMITTER: Carter CJ

PROVIDER: S-EPMC1172689 | biostudies-other | 1976 Jan

REPOSITORIES: biostudies-other

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