Unknown

Dataset Information

0

The role of mitochondria in modifying calcium-sensitive cytoplasmic metabolic activities. Modification of pyruvate kinase activity.


ABSTRACT: 1. The modification of pyruvate kinase activity in vitro was examined by altering the environmental [Mg(2+)]/[Ca(2+)] ratio with EDTA on the one hand and isolated rat liver mitochondria on the other. 2. Controlled additions of Ca(2+) and EDTA caused pyruvate kinase activity to be alternately and rapidly switched on and off. 3. By being able to accumulate Ca(2+) in preference to Mg(2+) rat liver mitochondria were able to alter the [Mg(2+)]/[Ca(2+)] ratio in the vicinity of pyruvate kinase and thereby modify the activity of this enzyme. 4. The possible role of mitochondria in modifying pyruvate kinase and other ion-sensitive cytoplasmic enzyme activities is discussed.

SUBMITTER: Meli J 

PROVIDER: S-EPMC1173777 | biostudies-other | 1972 Jun

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC6964030 | biostudies-literature
| S-EPMC1168533 | biostudies-other
| S-EPMC8395047 | biostudies-literature
| S-EPMC3129615 | biostudies-literature
| S-EPMC7198623 | biostudies-literature
| S-EPMC9009071 | biostudies-literature
| S-EPMC7214446 | biostudies-literature
| S-EPMC10706023 | biostudies-literature
| S-EPMC10050374 | biostudies-literature
| S-EPMC7511668 | biostudies-literature