Unknown

Dataset Information

0

The oxidation of Schiff bases of pyridoxamine with -oxo acids by manganous ions and peroxidase.


ABSTRACT: 1. Oxygen was taken up rapidly when pyruvate was added to mixtures of pyridoxamine and Mn(2+) ions after lag periods that were shortened by peroxidase (donor-hydrogen peroxide oxidoreductase, EC 1.11.1.7). 2. The total oxygen uptake was proportional to the pyridoxamine added and was accompanied by the disappearance of pyridoxamine; the pyruvate acted catalytically and hydrogen peroxide was not formed. 3. At pH6 more than half the pyridoxamine that disappeared was accounted for as pyridoxal and ammonia; it is suggested that the primary reaction is the oxidative deamination of the pyridoxamine. 4. Results were similar when alpha-oxobutyrate or glyoxylate were substituted for pyruvate, except that the reactions were slower and the yield of pyridoxal less. 5. The oxidative decarboxylations of alpha-oxoglutarate and phenylpyruvate are catalysed by Mn(2+) ions and these reactions are activated by peroxidase; pyridoxamine increased both the rates and total oxygen uptakes in these reactions, and ammonia was produced. 6. The lag periods in the oxidation of mixtures of pyridoxamine and alpha-oxo acids, catalysed by Mn(2+) ions, were also shortened by traces of colloidal manganese dioxide. 7. It is suggested that the activating effect of peroxidase depends on its catalysis of manganese oxidation.

SUBMITTER: Hill JM 

PROVIDER: S-EPMC1173821 | biostudies-other | 1972 Jul

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC1265015 | biostudies-other
| S-EPMC8756576 | biostudies-literature
| S-EPMC6271590 | biostudies-literature
| S-EPMC7474185 | biostudies-literature
| S-EPMC10171175 | biostudies-literature
| S-EPMC10867894 | biostudies-literature
| S-EPMC6269710 | biostudies-literature
| S-EPMC6271760 | biostudies-literature
| S-EPMC8252515 | biostudies-literature
| S-EPMC8685555 | biostudies-literature