Accumulation of untranslated lactose-specific messenger ribonucleic acid during catabolite repression in Escherichia coli.

ABSTRACT: When Escherichia coli K-12 Hfr.H was induced to synthesize beta-galactosidase in the presence of glucose, an untranslated lactose-specific mRNA (lac-mRNA), protected from decay, was found to accumulate progressively within the cells. The lac-mRNA accumulation was unaffected by the carbon source on which the cells had been grown before the induction. The amount of the lac-mRNA available for translation was affected by catabolite repression and 3':5'-cyclic AMP, but it remained unclear whether this was a direct effect on the formation of the lac-mRNA or a consequence of the effect on its translation.

SUBMITTER: Aboud M 

PROVIDER: S-EPMC1176765 | biostudies-other | 1971 Apr

REPOSITORIES: biostudies-other