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Interaction of dehydroheliotridine, a metabolite of heliotridine-based pyrrolizidine alkaloids, with native and heat-denatured deoxyribonucleic acid in vitro.


ABSTRACT: Dehydroheliotridine, a major pyrrolic metabolite of heliotridine-based pyrrolizidine alkaloids, interacted with both native and heat-denatured DNA in vitro under mildly acid conditions to form a soluble non-diffusible complex as shown by spectral data. The complex formed with heat-denatured DNA was more resistant than heat-denatured DNA alone to the combined action of deoxyribonuclease and phosphodiesterase. Analysis of the resistant fractions suggested the presence of at least one base not obtained from DNA alone.

SUBMITTER: Black DN 

PROVIDER: S-EPMC1179198 | biostudies-other | 1970 Jul

REPOSITORIES: biostudies-other

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