Phosphorus nuclear-magnetic-resonance studies of the transition-state analogue complex of creatine kinase.
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ABSTRACT: 31P nuclear-magnetic-resonance spectra of MgADP bound to creatine kinase in the presence of creatine NO3- ions show that there are two non-identical forms of the bound nucleotide. The sites have different affinities for the nucleotide. MgADP at the high-affinity site is in slow exchange (k less than 125s-1 at 27 degrees C) with free MgADP.
SUBMITTER: Milner-White EJ
PROVIDER: S-EPMC1183731 | biostudies-other | 1977 Dec
REPOSITORIES: biostudies-other
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