Iron-binding fragments from the N-terminal and C-terminal regions of human lactoferrin.
Ontology highlight
ABSTRACT: Digestion of lactoferrin with pepsin at pH3.0 gave an iron-binding half-molecule that represents the C-terminal part of the native protein. Tryptic or chymotryptic digestion of 30%-iron-saturated lactoferrin yielded the N- and C-terminal half molecules, which could be separated by DEAE-Sephadex chromatography. The N- and C-terminal fragments did not show any immunological cross-reaction. The carbohydrate of lactoferrin was distributed equally between the two fragments.
SUBMITTER: Bluard-Deconinck JM
PROVIDER: S-EPMC1183960 | biostudies-other | 1978 May
REPOSITORIES: biostudies-other
ACCESS DATA
