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Characterization of uterine heterogeneous nuclear ribonucleic acid and the effect of oestradiol-17 beta on its synthesis.

ABSTRACT: An early response to the administration of oestradiol-17 beta to immature rats is a dramatic stimulation in the synthesis of uterine hnRNA (heterogenous nuclear RNA). High-molecular-weight fractions of the hnRNA were purified and subfractionated on poly(U)-Sepharose into fractions that differed in their poly(A) content and their size profile on polyacrylamide gels. Oestrogen treatment of the rats stimulated the synthesis of all three fractions of high-molecular-weight hnRNA, but the kinetics of synthesis, degree of stimulation and size distribution of the newly synthesize RNA differed in each fraction.

SUBMITTER: Aziz S

PROVIDER: S-EPMC1185734 | biostudies-other | 1978 Jun

REPOSITORIES: biostudies-other

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The synthesis of ribonucleic acid in immature rat uterus responding to oestradiol-17 beta.

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The oestrogen-stimulated synthesis of heterogeneous nuclear ribonucleic acid in the uterus of immature rats.

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The use of rat liver nucleoplasm for the characterization of heterogeneous nuclear ribonucleic acid synthesis in vitro.

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