The preparation of stable enzyme-coenzyme complexes with endogenous catalytic activity.
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ABSTRACT: N6-(6-Aminohexylcarbamoylmethyl)-NAD+ was coupled to lactate dehydrogenase by using glutaraldehyde to form an active complex. The stability of this complex could be considerably improved by reduction with KBH4, although this treatment caused a partial decrease in specific activity. NAD+ was also coupled directly to the enzyme by this method. All of these complexes exhibited an intrinsic activity in the absence of exogenous NAD+.
SUBMITTER: Gacesa P
PROVIDER: S-EPMC1186378 | biostudies-other | 1979 Jan
REPOSITORIES: biostudies-other
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