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The partial sequence of two large peptides from the N-terminal half of heavy chains from normal rabbit immunoglobulin G.

ABSTRACT: The partial amino acid sequence of two large peptides is described. These were prepared from the N-terminal half of the heavy chain of immunoglobulin G from pooled normal rabbit serum by tryptic digestion after the in-amino groups of the lysine residues had been blocked with S-ethyl trifluorothioacetate. These peptides are believed to account for about 145 residues of fragment C-1, the N-terminal section of rabbit immunoglobulin G heavy chain prepared by cyanogen bromide cleavage. The evidence from the present paper and the preceding paper (Cebra, Givol & Porter, 1968) suggests that it may be possible to deduce a predominant amino acid sequence for most, if not all, of this section of the molecule.

SUBMITTER: Cebra JJ

PROVIDER: S-EPMC1198613 | biostudies-other | 1968 Mar

REPOSITORIES: biostudies-other

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Common peptides from the N-terminal half of heavy chain of immunoglobulin G from normal rabbit serum and a specific antibody.

Project description:Fragment C-1, the N-terminal half of the heavy chain of rabbit immunoglobulin G, was prepared by cyanogen bromide cleavage from the heavy chain of immunoglobulin G obtained both from the pooled serum of normal rabbits and from specific anti-dinitrophenyl antibody. Tryptic digestion of fragment C-1 after the lysine residues had been allowed to react with S-ethyl trifluorothioacetate led to the isolation of six peptides from inert immunoglobulin G and specific antibody that appear to account for most of this section of the heavy chain. This approach should make possible comparative sequence studies of the Fd section of the heavy chain from different allotypes and from specific antibodies.

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Allotypically related sequences in the Fd fragment of rabbit immunoglobulin heavy chains.

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| S-EPMC1177145 | biostudies-other

The N-terminal sequence of the heavy chain of rabbit immunoglobulin IgG.

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Variation in the N-terminal sequence of heavy chains of immunoglobulin G from rabbits of different allotype.

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| S-EPMC1198421 | biostudies-other

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Comparative studies of two types of bovine immunoglobulin G heavy chains.

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Intrachain disulphide bridges in immunoglobulin G heavy chains. The Fc fragment.

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