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Expression of hydrophilic surfactant proteins by mesentery cells in rat and man.

ABSTRACT: Human peritoneal dialysis effluent (PDE) contains a phosphatidylcholine-rich compound similar to the surfactant that lines lung alveoli. This material is secreted by mesothelial cells. Lung surfactant is also characterized by four proteins essential to its function. After having long been considered as lung-specific, some of them have been found in gastric and intestinal epithelial cells. To explore further the similarity between lung and peritoneal surfactants, we investigated whether mesothelial cells also produce surfactant proteins. We used rat transparent mesentery, human visceral peritoneum biopsies and PDE. Surfactant proteins were searched for after one- and two-dimensional SDS/PAGE and Western blotting. On a one-dimensional Western blot, bands at 38 and 66 kDa in rat mesentery, and at 38 and 66 kDa in human peritoneal mesothelial cells (in vivo and in vitro) and PDE, corresponded to monomeric and dimeric forms of lung surfactant protein A (SP-A). On two-dimensional Western blots, the 32 and 38 kDa spots in mesentery and PDE localized at the acidic pH appropriate to the SP-A monomer's isoelectric point. SP-D was also identified at the same 43 kDa molecular mass as in lung. SP-B was not detected in mesenteric samples. Expression of SP mRNA species was also assessed by reverse transcriptase-PCR, which was performed with specific primers of surfactant protein cDNA sequences. With primers of SP-A and SP-D, DNA fragments of the same size were amplified in lung and mesentery, indicating the presence of SP-A and SP-D mRNA species. These fragments were labelled by appropriate probes in a Southern blot. No amplification was obtained for SP-B. These results show that mesentery cells produce SP-A and SP-D, although they are of embryonic origin (mesodermal) and are different from those of the lung and digestive tract (endodermal) that secrete these surfactants.

SUBMITTER: Chailley-Heu B

PROVIDER: S-EPMC1218914 | biostudies-other | 1997 Nov

REPOSITORIES: biostudies-other

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Project description:The agricultural industry requires improved efficacy of sprays being applied to crops and weeds to reduce their environmental impact and increase financial returns. One way to improve efficacy is by enhancing foliar penetration. The plant leaf cuticle is the most significant barrier to agrochemical diffusion within the leaf. The importance of a mechanistic mathematical model has been noted previously in the literature, as each penetration experiment is dictated by its specific parameters, namely plant species, environmental conditions such as relative humidity and spray formulation including adjuvant addition. A mechanistic mathematical model has been previously developed by the authors, focusing on plant cuticle diffusion of calcium chloride through tomato fruit cuticles including pore swelling, ion binding and evaporation, along with the ability to vary the active ingredient concentration and type, relative humidity and plant species. Here we further develop this model to include adjuvant effects as well as the hygroscopic nature of deliquescent ionic solutions with evaporation on the cuticle surface. These modifications to a penetration and evaporation model provide a novel addition to the literature and allow the model to be applied to many types of evaporating ionic hygroscopic solutions on many types of substrates, not just plant cuticles. We validate our theoretical model results against appropriate experimental data, discuss key sensitivities and relate theoretical predictions to physical mechanisms. The important governing mechanisms influencing surfactant enhanced penetration of ionic active through plant cuticles were found to be aqueous pore radius, pore density, cuticle thickness and initial contact angle of the applied droplet; ion binding, relative humidity and evaporation including hygroscopic water absorption parameters for point of deliquescence. The sensitivity analysis indicated surfactants increase penetration by changing the point of deliquescence of a solution, which alters the water absorption and the initial contact angle, which alters the number of pores under the droplet. The results of the validation and sensitivity analysis imply that this model accounts for many of the mechanisms governing penetration in plant cuticles.

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Expression of hydrophilic surfactant proteins by mesentery cells in rat and man.

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