The separation of the alpha-chains of collagen by free-flow electrophoresis.
Ontology highlight
ABSTRACT: 1. The separation of the alpha-components of codfish-skin and calf-skin collagen from the higher-molecular-weight components was accomplished by gel filtration with Bio-Gel P-300 resin in 5m-lithium chloride and at pH7.4. 2. From this fraction three distinct alpha-components, corresponding to the alpha1-, alpha2- and alpha3- chains of collagen, were isolated by free-flow electrophoresis in 6m-urea at 4 degrees . At the temperatures and pH values used there was no detectable degradation of the alpha-chains.
SUBMITTER: Francois CJ
PROVIDER: S-EPMC1270221 | biostudies-other | 1967 Jan
REPOSITORIES: biostudies-other
ACCESS DATA