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The separation of the alpha-chains of collagen by free-flow electrophoresis.


ABSTRACT: 1. The separation of the alpha-components of codfish-skin and calf-skin collagen from the higher-molecular-weight components was accomplished by gel filtration with Bio-Gel P-300 resin in 5m-lithium chloride and at pH7.4. 2. From this fraction three distinct alpha-components, corresponding to the alpha1-, alpha2- and alpha3- chains of collagen, were isolated by free-flow electrophoresis in 6m-urea at 4 degrees . At the temperatures and pH values used there was no detectable degradation of the alpha-chains.

SUBMITTER: Francois CJ 

PROVIDER: S-EPMC1270221 | biostudies-other | 1967 Jan

REPOSITORIES: biostudies-other

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