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Chemical analysis of acetylated bovine growth hormone, a growth-hormone inhibitor.

ABSTRACT: Purified bovine growth hormone was acetylated with acetic anhydride under various conditions. Acetylated bovine growth hormone prepared in anhydrous acetic anhydride at 43 degrees for 5min. inhibited the growth-promoting response of unmodified bovine growth hormone in hypophysectomized rats. This acetylated bovine growth hormone contained 18 total acetyl groups/mol., which included 12 N(in)-acetyl and 6-8 hydroxylamine-reactive acetyl groups (acetylated aliphatic hydroxy amino acids and 2-3 O-acetyltyrosine residues). Deacetylation of acetylated bovine growth hormone with hydroxylamine at pH9.5 destroyed its ability to inhibit the growth-promoting activity of bovine growth hormone.

SUBMITTER: Oikawa A

PROVIDER: S-EPMC1271236 | biostudies-other | 1967 Sep

REPOSITORIES: biostudies-other

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