Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein.
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ABSTRACT:
SUBMITTER: Bird LE
PROVIDER: S-EPMC146783 | biostudies-other | 1997 Jul
REPOSITORIES: biostudies-other
Publications
Characterization and crystallization of the helicase domain of bacteriophage T7 gene 4 protein.
Bird L E LE Hâkansson K K Pan H H Wigley D B DB
Nucleic acids research 19970701 13
Limited proteolysis of bacteriophage T7 primase/helicase with endoproteinase Glu-C produces several proteolytic fragments. One of these fragments, which is derived from the C-terminal region of the protein, was prepared and shown to retain helicase activity. This result supports a model in which the gene 4 proteins consist of functionally separable domains. Crystals of this C-terminal fragment of the protein have been obtained that are suitable for X-ray diffraction studies. ...[more]
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