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Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.

ABSTRACT: A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI, shares 66% identity and 76% similarity with M. Cvi JI, the only other cytosine-5-DNA methyltransferase cloned from a Chlorella virus. The short, frequently occurring recognition sequence of the new methyltransferase will be very useful for in vivo chromatin structure studies in both yeast and higher organisms.

SUBMITTER: Xu M

PROVIDER: S-EPMC147793 | biostudies-other | 1998 Sep

REPOSITORIES: biostudies-other

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Cloning, characterization and expression of the gene coding for a cytosine-5-DNA methyltransferase recognizing GpC.

Xu M M Kladde M P MP Van Etten J L JL Simpson R T RT

Nucleic acids research 19980901 17

A novel gene encoding a cytosine-5-DNA methyltransferase recognizing the dinucleotide GpC was cloned from Chlorella virus NYs-1 and expressed in both Escherichia coli and Saccharomyces cerevisiae . The gene was sequenced and a predicted polypeptide of 362 amino acids with a molecular weight of 41.903 kDa was identified. The protein contains several amino acid motifs with high similarity to those of other known 5-methylcytosine-forming methyltransferases. In addition, this enzyme, named M. Cvi PI ...[more]

PMID: 9705505

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