Unknown

Dataset Information

0

Modulation of glycine receptors in retinal ganglion cells by zinc.


ABSTRACT: Effects of zinc, an endogenous neuromodulator in the central nervous system, on glycine receptors (GlyRs) in retinal ganglion cells were investigated by using the whole-cell voltage-clamp technique. Zn2+ at low concentration (<2 microM) potentiated the glycine-induced chloride current and at higher concentration (>10 microM) suppressed it. This biphasic regulatory action of zinc acted selectively on the fast component of the glycine-induced current mediated by the strychnine-sensitive GlyRs, but not on the slow component mediated by the 5,7-dichlorokynurenic acid-sensitive GlyRs. Dose-response studies showed that 1 microM Zn2+ increased the maximum glycine response (I approximately) and shifted the EC50 to the left, suggesting that Zn2+ at low concentrations acts as an allosteric activator of the strychnine-sensitive GlyRs. Zn2+ at a concentration of 100 microM did not alter I approximately and shifted the EC50 to the right, indicating that Zn2+ at high concentrations acts as a competitive inhibitor of the GlyRs. Physiological functions of zinc modulation of GlyRs in retinal ganglion cells are discussed.

SUBMITTER: Han Y 

PROVIDER: S-EPMC15925 | biostudies-other | 1999 Mar

REPOSITORIES: biostudies-other

Similar Datasets

| S-EPMC4928062 | biostudies-literature
| S-EPMC9206055 | biostudies-literature
| S-EPMC5561962 | biostudies-other
| S-EPMC5306473 | biostudies-other
| S-EPMC8030271 | biostudies-literature
| S-EPMC4998662 | biostudies-literature
| S-EPMC7393603 | biostudies-literature
| S-EPMC9005408 | biostudies-literature
| S-EPMC4919579 | biostudies-literature
| S-EPMC7056773 | biostudies-literature