Ontology highlight
ABSTRACT:
SUBMITTER: Yang B
PROVIDER: S-EPMC1679691 | biostudies-other | 2006 Dec
REPOSITORIES: biostudies-other
Molecular biology of the cell 20061011 12
Sir protein spreading along chromosomes and silencing in Saccharomyces cerevisiae requires the NAD+-dependent histone deacetylase activity of Sir2p. We tested whether this requirement could be bypassed at the HM loci and telomeres in cells containing a stably expressed, but catalytically inactive mutant of Sir2p, sir2-345p, plus histone mutants that mimic the hypoacetylated state normally created by Sir2p. Sir protein spreading was rescued in sir2-345 mutants expressing histones in which key lys ...[more]