Sir protein-independent repair of dicentric chromosomes in Saccharomyces cerevisiae.
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ABSTRACT:
SUBMITTER: McCleary DF
PROVIDER: S-EPMC5025274 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Publications
Sir protein-independent repair of dicentric chromosomes in Saccharomyces cerevisiae.
McCleary David F DF Steakley David Lee DL Rine Jasper J
Molecular biology of the cell 20160727 18
Sir2 protein has been reported to be recruited to dicentric chromosomes under tension, and such chromosomes are reported to be especially vulnerable to breakage in sir2Δ mutants. We found that the loss of viability in such mutants was an indirect effect of the repression of nonhomologous end joining in Sir(-) mutants and that the apparent recruitment of Sir2 protein to chromosomes under tension was likely due to methodological weakness in early chromatin immunoprecipitation studies. ...[more]
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