Ontology highlight
ABSTRACT:
SUBMITTER: Seyfried M
PROVIDER: S-EPMC178423 | biostudies-other | 1996 Oct
REPOSITORIES: biostudies-other
Seyfried M M Daniel R R Gottschalk G G
Journal of bacteriology 19961001 19
The genes encoding coenzyme B12-dependent glycerol dehydratase of Citrobacter freundii were cloned and overexpressed in Escherichia coli. The B12-free enzyme was purified to homogeneity. It consists of three types of subunits whose N-terminal sequences are in accordance with those deduced from the open reading frames dhaB, dhaC, and dhaE, coding for subunits of 60,433 (alpha), 21,487 (beta), and 16,121 (gamma) Da, respectively. The enzyme complex has the composition alpha2beta2gamma2. Amino acid ...[more]