Binding of a novel host factor to the pT181 plasmid replication enhancer.
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ABSTRACT:
SUBMITTER: Zhang Q
PROVIDER: S-EPMC178748 | biostudies-other | 1997 Feb
REPOSITORIES: biostudies-other
Publications
Binding of a novel host factor to the pT181 plasmid replication enhancer.
Zhang Q Q Soares de Oliveira S S Colangeli R R Gennaro M L ML
Journal of bacteriology 19970201 3
Replication enhancers are cis-acting genetic elements that stimulate the activity of origins of DNA replication. The enhancer found in plasmid pT181 of Staphylococcus aureus, called cmp, functions at a distance of 1 kb from the origin of DNA replication to stimulate the interaction between the replication initiation protein and the origin. DNA encoding cmp-binding activity was isolated by screening an expression library of S. aureus DNA in Escherichia coli, and a novel gene, designated cbf1, was ...[more]
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