Dataset Information

The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.

ABSTRACT: The ggtA gene was sequenced during the analysis of a mutant of Synechocystis sp. strain PCC 6803 with impaired salt tolerance. It showed striking sequence similarities to ATP-binding proteins of binding-protein-dependent transport systems (ABC transporters). Mutants of ggtA and three neighboring reading frames were constructed by inserting an aphII gene cassette and were physiologically and genetically characterized. The ggtA insertion mutant lost its glucosylglycerol (GG) uptake ability, but its salt tolerance did not change. Therefore, it was concluded that active transport of the osmoprotective compound GG in Synechocystis is mediated by an ABC transporter. The genes for the GG-specific ABC transporter are not organized in an operon as usually found for comparable transporters, since the other insertion mutants showed normal GG transport activity. After cultivation of the ggtA mutant at high salt concentrations, significant amounts of GG were found in the cultivation medium, indicating that GG transport is mainly necessary for recovery of GG leaked through the cytoplasmic membrane. The Northern blot technique revealed increased transcription of the ggtA gene in cells adapted to higher salt concentrations, whereas in cells from basal medium, its transcription was weak.

SUBMITTER: Hagemann M

PROVIDER: S-EPMC178752 | biostudies-other | 1997 Feb

REPOSITORIES: biostudies-other

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The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.

Hagemann M M Richter S S Mikkat S S

Journal of bacteriology 19970201 3

The ggtA gene was sequenced during the analysis of a mutant of Synechocystis sp. strain PCC 6803 with impaired salt tolerance. It showed striking sequence similarities to ATP-binding proteins of binding-protein-dependent transport systems (ABC transporters). Mutants of ggtA and three neighboring reading frames were constructed by inserting an aphII gene cassette and were physiologically and genetically characterized. The ggtA insertion mutant lost its glucosylglycerol (GG) uptake ability, but it ...[more]

PMID: 9006025

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Project description:In recent years, there has been an increased interest in the research and development of sustainable alternatives to fossil fuels. Using photosynthetic microorganisms to produce such alternatives is advantageous, since they can achieve direct conversion of carbon dioxide from the atmosphere into the desired product, using sunlight as the energy source. Squalene is a naturally occurring 30-carbon isoprenoid, which has commercial use in cosmetics and in vaccines. If it could be produced sustainably on a large scale, it could also be used instead of petroleum as a raw material for fuels and as feedstock for the chemical industry. The unicellular cyanobacterium Synechocystis PCC 6803 possesses a gene, slr2089, predicted to encode squalene hopene cyclase (Shc), an enzyme converting squalene into hopene, the substrate for forming hopanoids. Through inactivation of slr2089 (shc), we explored the possibility to produce squalene using cyanobacteria. The inactivation led to accumulation of squalene, to a level over 70 times higher than in wild type cells, reaching 0.67 mg OD750(-1) L(-1). We did not observe any significant growth deficiency in the Δshc strain compared to the wild type Synechocystis, even at high light conditions, suggesting that the observed squalene accumulation was not detrimental to growth, and that formation of hopene by Shc is not crucial for growth under normal conditions, nor for high-light stress tolerance. Effects of different light intensities and growth stages on squalene accumulation in the Δshc strain were investigated. We also identified a gene, sll0513, as a putative squalene synthase in Synechocystis, and verified its function by inactivation. In this work, we show that it is possible to use the cyanobacterium Synechocystis to generate squalene, a hydrocarbon of commercial interest and a potential biofuel. We also report the first identification of a squalene hopene cyclase, and the second identification of squalene synthase, in cyanobacteria.

Dataset Information

The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.

Publications

The ggtA gene encodes a subunit of the transport system for the osmoprotective compound glucosylglycerol in Synechocystis sp. strain PCC 6803.

Similar Datasets

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