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Modification of retroviral RNA by double-stranded RNA adenosine deaminase.

ABSTRACT: In this report, we describe a recombinant provirus generated during in vitro passage that contains a short region of adenosine-to-guanosine hypermutation. The hypermutated region is restricted to complementary sequences present in the recombinant provirus. We propose that a duplex was formed in the recombinant RNA prior to reverse transcription. This duplex was a substrate for double-stranded RNA adenosine deaminase, an activity found in all cells examined that deaminates A in double-stranded RNA, converting it to inosine, which is further converted to a guanosine by reverse transcription. It appears that cis viral sequences facilitated the A-->G transitions.

SUBMITTER: Hajjar AM

PROVIDER: S-EPMC189466 | biostudies-other | 1995 Sep

REPOSITORIES: biostudies-other

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Modification of retroviral RNA by double-stranded RNA adenosine deaminase.

Hajjar A M AM Linial M L ML

Journal of virology 19950901 9

In this report, we describe a recombinant provirus generated during in vitro passage that contains a short region of adenosine-to-guanosine hypermutation. The hypermutated region is restricted to complementary sequences present in the recombinant provirus. We propose that a duplex was formed in the recombinant RNA prior to reverse transcription. This duplex was a substrate for double-stranded RNA adenosine deaminase, an activity found in all cells examined that deaminates A in double-stranded RN ...[more]

PMID: 7543593

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2019-12-22 | GSE131658 | GEO

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