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Mapping and characterization of the origin of DNA replication of porcine circovirus.

ABSTRACT: The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins.

SUBMITTER: Mankertz A

PROVIDER: S-EPMC191374 | biostudies-other | 1997 Mar

REPOSITORIES: biostudies-other

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Mapping and characterization of the origin of DNA replication of porcine circovirus.

Mankertz A A Persson F F Mankertz J J Blaess G G Buhk H J HJ

Journal of virology 19970301 3

The origin of DNA replication of porcine circovirus (PCV) was mapped to a 111-bp fragment. On top of a hairpin, a nonanucleotide (TAGTATTAC) homologous to nonanucleotides of other viruses was identified. Mutation of this element abolishes replication. PCV may be related to a virus family characterized by single-stranded circular DNA genomes, rolling-circle replication, and homology of their rep proteins. ...[more]

PMID: 9032401

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| S-EPMC4283266 | biostudies-literature

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